Extended Data Fig. 3: Flow cytometry analysis of cell-surface GLUT1 expression in efferocytic macrophages.
From: PFKFB2-mediated glycolysis promotes lactate-driven continual efferocytosis by macrophages
BMDMs were incubated with PKH67-labeled apoptotic cells (ACs) for 45 min, rinsed and harvested 1 h later for flow cytometric analysis of cell-surface GLUT1 using an APC-conjugated GLUT1 antibody. a, Gating strategy for AC− and AC+ macrophages, with PKH67 detected in the FITC channel. b, The mean fluorescent intensity (MFI) of cell-surface GLUT1 based on the flow cytometric data.
