Fig. 5: G6PDH, through regulation of PDI, maintains collagen properties. | Nature Metabolism

Fig. 5: G6PDH, through regulation of PDI, maintains collagen properties.

From: The pentose phosphate pathway controls oxidative protein folding and prevents ferroptosis in chondrocytes

Fig. 5

a, Hydroxyproline (OH-proline) levels in differentiated hypoxic chondrocytes from G6pdhchon+ and G6pdhchon− mice (G6pdhchon+, n = 6; G6pdhchon−, n = 5; unpaired two-sided Student’s t-test vs G6pdhchon+). b,c, OH-proline content released in supernatant or remaining in neonatal growth plates (GP) from G6pdhchon+ and G6pdhchon− mice after incubation with MMP9 (b) or MMP13 (c) (n = 4; unpaired two-sided Student’s t-test vs G6pdhchon+). d, Safranin O (SafO) staining of the primary spongiosa of neonatal G6pdhchon+ and G6pdhchon− mice with the quantification of SafO-positive area (n = 4, *: P = 0.02, unpaired two-sided Student’s t-test vs G6pdhchon+). Scale bar, 100 µm. The data are presented as means ± s.d.

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