Extended Data Fig. 4: Maternal exercise-mediated improvement of offspring metabolic health is blunted by WATs-specific knockdown of Serpina3c in dams.
From: Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3C
Mice were treated as indicated in Fig. 4. a, Glucose concentration during the i.p. GTT in male and female offspring after 7 weeks of HFD feeding. b, HE staining of iWAT and VAT from male and female offspring, respectively. VAT includes male eWAT and female POAT. Scale bar: 50 μm. c,d, Quantitative analyses of adipocyte size distribution of iWAT and VAT from male (c) and female (d) offspring. e,f, Liver TG levels from female (e) and male (f) offspring. g,h, Quantitative analysis of p-AKT/AKT ratio in Fig. 4i. n = 3 mice for each group. i,j, Quantitative analyses of the F4/80 IF staining in Fig. 4l. k, Flow cytometry for detecting CD11b+/F4/80+ macrophages from CD45+ cells in SVF isolated from offspring iWAT. For statistical analyses, two-way ANOVA with Bonferroni’s post hoc tests were performed in e-j. All values are represented as means with error bars representing S.D. n = 6 for each group unless otherwise mentioned. i.p., intraperitoneal injection; GTT, glucose tolerance test; TG, triglyceride; IF, immunofluorescence.
