Extended Data Fig. 5: The impact of WATs-specific overexpression of Serpina3c on the dams and fetuses.
From: Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3C
Mice were treated as in Fig. 5. a, Body weight change curve and body weight gain during pregnancy of the dams. b, The serum Serpina3c level at gestation day 18.5 (d18.5) in dams. c, Serpina3c mRNA levels in iWAT, POAT, liver and Gas of dams at gestation d18.5. d, The serum Serpina3c level in fetuses at E18.5. e, f, Western blotting (e) was conducted to detect Serpina3c protein levels in placenta from dams, as well as in fetal liver and inguinal region. Quantitative analysis (f) by Serpina3c/HSP90 ratio was performed. n = 3 mice for each group. g,h, Western blotting(g) was conducted to detect Serpina3c protein levels in iWAT and POAT from dams after the weaning period, and quantitative analysis (h) by Serpina3c/HSP90 ratio was performed. n = 3 mice for each group. For statistical analyses, two-way ANOVA with Bonferroni’s post hoc tests were performed in a,b,h, and unpaired two-tailed Student’s t tests were performed in c,d,f,. All values are represented as means with error bars representing S.D. n = 6 for each group unless otherwise mentioned. E18.5, embryonic day 18.5; Gas, gastrocnemius.
