Extended Data Fig. 6: Maternal exercise-mediated improvement of offspring metabolic health can be mimicked by WATs-specific overexpression of Serpina3c in dams.
From: Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3C
Mice were treated as indicated in Fig. 5. a,b, Glucose concentration during an i.p. GTT in male (a) and female (b) offspring after 7 weeks of HFD feeding. c, HE staining of iWAT and VAT from male and female offspring, respectively, Scale bar: 50 μm. d,e, Quantitative analyses of adipocyte size distribution of iWAT and VAT from male (d) and female (e) offspring. f,g, Quantitative analysis of p-AKT/AKT ratio in Fig. 5h, with (f) for male offspring and (g) for female offspring. n = 3 mice for each group. h,i, Quantitative analyses of the IF staining of F4/80 in Fig. 5l. j, Flow cytometry for detecting CD11b+/F4/80+ macrophages from CD45+ cells in SVF isolated from offspring iWAT. For statistical analyses, two-way ANOVA with Bonferroni’s post hoc tests were performed in f-i. All values are represented as means with error bars representing S.D. n = 6 for each group unless otherwise mentioned. i.p., intraperitoneal injection; GTT, glucose tolerance test; SVF, stromal vascular fraction; IF, immunofluorescence.
