Extended Data Fig. 9: The effects of Klk1 and Klk1b26 on the DNA methylation level of the Klf4 gene promoter in adipocytes, the effects of Serpina3c on the DNA methylation level of the Cebpb gene promoter in adipocytes and the effects of Serpina3c knockdown or overexpression on glucose uptake and inflammation-related genes expression in maternal WATs.
From: Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3C
a, Activity of TETs in 3T3-L1 adipocytes after being treated with 50 ng/ml rKlk1 or rKlk1b26 for 6 h. b, 5mc and 5hmc enrichment on Klf4 gene promoter in 3T3-L1 adipocytes after being treated by 50 ng/ml rKlk1 or rKlk1b26 for 24 h. c, 5mc and 5hmc enrichment on mouse Cebpb gene promoter in 3T3-L1 adipocytes treated with 20 ng/ml rSerpina3c for 24 h. d,e, 2-NBDG uptake was determined in iWAT and POAT from the chow diet-fed sedentary dams (at gestation d18.5) with/out Serpina3c knockdown in the WATs (d) and with/out Serpina3c overexpression in the WATs (e). 2-NBDG uptake was measured after in vitro stimulation of the iWAT and POAT with/out insulin. f,g, The mRNA levels of the indicated genes were determined by RT-qPCR in iWAT and POAT from the chow diet-fed sedentary dams (at gestation d18.5) with/out Serpina3c knockdown in the WATs (f) and with/out Serpina3c overexpression in the WATs (g). For statistical analyses in a,b, one-way ANOVA tests plus Bonferroni’s post hoc tests were performed, and unpaired two-tailed Student’s t tests were performed in c-g. All values are represented as means with error bars representing S.D. n = 6 for each group. rKlk1, recombinant Klk1 protein; rKlk1b26, recombinant Klk1b26 protein; WATs, white adipose tissues.
