Extended Data Fig. 3: Validation of ‘TRAP’ed motion-specific neurons via c-fos immunostaining.
From: Vestibular neurons link motion sickness, behavioural thermoregulation and metabolic balance in mice
(a) Schematic timeline of TRAP protocol coupled with c-fos immnuostaining. (b) In the left panel, tdTomato-labeled neurons refer to ‘TRAP’ed motion-specific neurons, and c-fos positive cells indicate neurons were activated after mice being subjected to a second-round of motion. Percentage of colocalization between tdTomato-labeled neurons and c-fos positive cells in each specific brain region was shown in the right panel. n = 3 mice. Data are expressed as mean ± standard errors of the mean. Arrows point to co-localized cells between tdTomato and c-fos positive cells. (Relative to Fig. 2).
