Extended Data Fig. 7: Lung stem cell growth in vitro, H&E and MALDI glycogen imaging of different mouse models.
From: Glycogen drives tumour initiation and progression in lung adenocarcinoma
a, Schematic of isolating bronchoalveolar stem cells and differentiation to bronchiolar and alveolar organoids in matrigel. b, Representative bright field images of bronchiolar and alveolar organoids derived from WT and laforin−/− (LKO) animals. c, Number of colonies and colony size from bronchiolar and alveolar organoids derived from WT and laforin−/− (LKO) animals. Values are presented as mean +/− s.e.m. p-values were calculated using two-tailed t-test. d, Additional representative H&E images and MALDI imaging of an adjacent tissue section showing tumor formation and glycogen levels LSL-KrasG12D/p53fl/fl (KP) and LSL-KrasG12D/p53fl/fl /LKO(KPL) (n = 3 each). e, Additional representative H&E images and MALDI imaging of an adjacent tissue section showing tumor formation and glycogen levels LSL-KrasG12D/p53fl/fl (KP); LSL-KrasG12D/p53fl/fl/Gysfl/fl (KPG); LSL-KrasG12D/p53fl/fl/Gysfl/fl:Vehicle (KPG:V); LSL-KrasG12D/p53fl/fl/Gysfl/fl:Combination diet (KPG/C)animals (n = 2 each). f, Distribution of tumor grades across KP and KPL cohorts of mice (n = 3 mice; mean +/− s.e.m. p-values indicated; one-way ANOVA adjusted for Tukey’s multiple comparisons). g. Kaplan Meier survival analysis for mice in different groups. Log-rank test stated.
