Extended Data Fig. 8: The entire mass isotopologue distribution (MID) of glucose from [U-13C] pyruvate or glutamine. | Nature Metabolism

Extended Data Fig. 8: The entire mass isotopologue distribution (MID) of glucose from [U-13C] pyruvate or glutamine.

From: Spatial hepatocyte plasticity of gluconeogenesis during the metabolic transitions between fed, fasted and starvation states

Extended Data Fig. 8: The entire mass isotopologue distribution (MID) of glucose from [U-13C] pyruvate or glutamine.The alternative text for this image may have been generated using AI.

(a) Steady state gluconeogenic time course experiment from [U-13C]-pyruvate using total hepatocytes isolated from 24 h fasted mice. N = 3 mice. Glucose isotopologues with one 13C to six 13C carbons (M1 thru M6 isotopologues) are shown. Data are presented as mean values + SD. (b) Fed, fasting and starvation response of glucose isotopologues with one 13C to six 13C carbons (M1 thru M6 isotopologues) derived from [U-13C]-pyruvate using pericentral and periportal isolated hepatocytes. N = 4 for all conditions except 0hPC, 8hPP, 16hPP, and 24hPC (N = 3). Data are presented as mean values + SD. (c) Fasted 24 hr response of glucose isotopologues with one to three 13C carbons (% enrichment) derived from [U-13C]-glutamine comparing pericentral vs. periportal isolated hepatocytes using Control vs GLS2KO mice 24 h mice. N = 3 mice per condition. Data are presented as mean values + SD.

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