Extended Data Fig. 5: qPCR and Western blotting verified the accuracy of the transcriptome results.
a, Fifteen upregulated DEGs from the transcriptome in the P. berghei ANKA + KD group were randomly selected, and fragments per kilobase of transcript per million fragments mapped (FPKM) values were used as indicators to measure the level of gene expression (n = 6, Unpaired two-tailed Student’s t-tests). b, Five downregulated DEGs from the transcriptome in the P. berghei ANKA + KD group were randomly selected, with FPKM used as an indicator to measure the level of gene expression (n = 6, Unpaired two-tailed Student’s t-tests). c, Western blot results showing that the expression of DYNLL1 and tubulin, which are associated with the P. berghei ANKA cytoskeleton, was upregulated and that the expression of RPL7a and RPL30, which are related to the biosynthesis of ribosomes pathway, were downregulated in P. berghei ANKA after KD treatment. Representatives of 3 independently repeated times of each experiment with similar results. d-g, The relative expression levels of 20 DEGs were consistent with the transcriptome results (n = 3, Unpaired two-tailed Student’s t-tests). Data are presented as means ± SEMs (a and b).
