Extended Data Fig. 7: ACSL4 in fibroblast sensitizes epithelial ferroptosis through secreted PUFA.
From: Fibroblast lipid metabolism through ACSL4 regulates epithelial sensitivity to ferroptosis in IBD
a, Cell death (%) of NCM-460 cell treated with CM from ACSL4-Con and ACSL4-OE fibroblast (n = 3 for each group). b, Cell death (%) of NCM-460 cell treated with Control (0.5% DMSO), aqueous phase and lipid phase metabolite extraction from ACSL4-OE fibroblast CM, with and without liproxstatin-1 (1 μM) (n = 3 for each group). c, Cell death (%) of NCM-460 cell treated with RSL3 (0.5 μM), Control (BSA 5 μM), AA (20 μM), EPA (20 μM) and DHA (20 μM) with and without liproxstatin-1 (1 μM) (n = 3 for each group). Data are shown as mean ± SEM, statistical analysis was performed using two-tailed unpaired T-test (for two column comparison) or 2-way ANOVA (for groups comparison). All experiments were repeated at least twice independently, with similar results.
