Extended Data Fig. 4: Deletion of MondoA shows minimal effect in T cell homeostasis, related to Fig. 5. | Nature Metabolism

Extended Data Fig. 4: Deletion of MondoA shows minimal effect in T cell homeostasis, related to Fig. 5.

From: Targeting MondoA–TXNIP restores antitumour immunity in lactic-acid-induced immunosuppressive microenvironment

Extended Data Fig. 4: Deletion of MondoA shows minimal effect in T cell homeostasis, related to Fig. 5.

(a) Flow cytometric gating strategy of TCRβ+CD8+ T cells, TCRβ+CD4+ T cells and TCRβ+CD4+FOXP3+ Treg cells in the spleen of mice. (b-d) Flow cytometric analysis of proportions of CD4+ T cells (b), CD8+ T cells (c) and Treg cells (d) in the spleen, mesenteric and peripheral lymph nodes (mLNs and pLNs) from 8-week-old WT and MondoAfl/flCd4-Cre mice. (e, f) Flow cytometric analysis of the percentage of CD4− CD8− (DN), CD4+ CD8+ (DP), CD4+ CD8− (SP-CD4) and CD4− CD8+ (SP-CD8) in the thymus of 8-week-old WT and MondoAfl/flCd4-Cre mice. (g, h) Expression of CD62L and CD44 in CD4+ T cells (g) and CD8+ T cells (h) in the spleen, mLNs and pLNs of 8-week-old WT and MondoAfl/flCd4-Cre mice. (i, j) Flow cytometric analysis of the percentage of Ki67-expressing CD4+ T cells (i) and CD8+ T cells (j) in the spleen, mLNs and pLNs from 8-week-old WT and MondoAfl/flCd4-Cre mice. (k) Flow cytometric analysis of the percentage of IFN-γ and TNF producing CD8+ T cells in the spleen, mLNs and pLNs from 8-week-old WT and MondoAfl/flCd4-Cre mice. The data were representative of three independent experiments and presented as mean ± s.e.m. Statistical analyses were performed using two-way ANOVA followed by Sidak’s multiple comparisons test (b-k). ns, not significant.

Source data

Back to article page