Fig. 6: Meteorin is increased in hepatocytes in MASH liver.
From: A non-apoptotic caspase-8–meteorin pathway in hepatocytes promotes MASH fibrosis
a, METRN mRNA and meteorin protein were assayed in the livers of individuals with or without MASH. Data are presented as mean ± s.e.m.; *P = 0.029, unpaired two-tailed Student’s t-test; normal, n = 6; MASH, n = 6. b, METRN mRNA levels in livers of healthy individuals and those with MASLD, based on the RNA-seq dataset GSE126848. Data are mean ± s.e.m.; *P = 0.012, unpaired two-tailed Student’s t-test; healthy, n = 14; MASLD, n = 31. a.u., arbitrary units. c, Meteorin protein was increased in the livers of mice fed the FPC diet for 16 weeks, the HF-CDAA diet for 8 weeks or the ALIOS diet for 36 weeks. d, Meteorin immunoblot and quantification of liver extracts from C57BL/6J mice fed chow or the FPC diet for 8 or 16 weeks. Data are mean ± s.e.m.; **P = 0.0061, one-way ANOVA; chow, n = 3, 8 weeks FPC, n = 4, 16 weeks FPC, n = 4. e, Meteorin immunofluorescence in liver sections of normal and MASH human livers. Scale bar, 50 μm. Arrows, an example of a meteorin+HSA+ hepatocyte in each sample, highlighting the higher staining intensity of meteorin in MASH liver, which is quantified as the MFI. Arrowheads, meteorin+HSA− cells. The MFI for hepatocyte meteorin is presented as mean ± s.e.m.; ****P < 0.0001, unpaired two-tailed Student’s t-test; normal, n = 4; MASH, n = 4. f, Meteorin protein was assayed in primary hepatocytes from people with or without MASH.
