Extended Data Fig. 5: Double KO of SLC25A16 (A16) and SLC25A42 (A42) in K562 cells leads to an impairment of TCA cycle and lipid catabolism.
a, Isotopologue abundance of citrate/isocitrate, α-ketoglutarate, succinate, fumarate, malate, and aspartate in ctrl, A42 KO, A16 KO, and A16/A42 DKO K562 cells with [U-13C5]-glutamine tracing (2 mM, 2 hrs), with naturally occurring 13C abundance correction. b, Oxygen consumption rate (OCR) trace for A16/A42 DKO K562 cells during Seahorse fatty acid oxidation stress test with palmitate as the sole fuel source (n = 5). All data are expressed as mean ± SD and n = 3 if not otherwise indicated. Statistical significance was calculated using one-way ANOVA followed by Dunnett’s multiple comparisons test (a). Significance level was indicated as **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns p > 0.05.
