Fig. 5: A16/A42 DKO leads to a specific depletion of short-chain acyl-CoAs within the K562 mitochondria. | Nature Metabolism

Fig. 5: A16/A42 DKO leads to a specific depletion of short-chain acyl-CoAs within the K562 mitochondria.

From: Cellular pan-chain acyl-CoA profiling reveals SLC25A42/SLC25A16 in mitochondrial CoA import and metabolism

Fig. 5: A16/A42 DKO leads to a specific depletion of short-chain acyl-CoAs within the K562 mitochondria.

a, Mitochondrial acyl-CoA profiling in Ctrl and A16/A42 DKO K562 cells (y axis in log10 scale), highlighting a significant decrease of mitochondria-enriched short-chain acyl-CoAs in the DKO mitochondria. b, Representative acyl-CoAs from a, including significantly decreased dpCoASH and free CoASH, mitochondria-enriched short-chain acyl-CoAs (propionyl-CoA, succinyl/MMA-CoA, C5:0-CoA), cytosolic-enriched acyl-CoAs (acetyl-CoA, malonyl-CoA, (iso)butyryl-CoA) and long-chain C16:0-CoA in Ctrl and A16/A42 DKO mitochondria (y axis in linear scale). c, Whole-cell acyl-CoA profiling in Ctrl and A16/A42 DKO K562 cells (y axis in log10 scale). Data are expressed as scatter dot plots with mean (a, c) or mean ± s.d. (b); n = 3. Statistical significance was calculated using a two-tailed Student’s t-test. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05; ns, P > 0.05.

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