Extended Data Fig. 8: Lac-Phe does not activate POMC neurons.
From: Lac-Phe induces hypophagia by inhibiting AgRP neurons in mice
(a) A representative current-clamp trace showing effects of Lac-Phe (5 μM, 5 s puff) on a POMC neuron in POMC-CreERT2/Rosa26-LSL-tdTomato mice. (b-c) Temporal changes in the resting membrane potential (b) and firing frequency (c) of POMC neurons in the ARH in response to vehicle or 5 μM Lac-Phe (puff 5 s). (d) Representative microscopic images showing β-endorphin immunofluorescence (green) in tdTomato-labelled ARH neurons (red) in male TRAP2/Rosa26-LSL-tdTomato mice (3 months of age) following Lac-Phe TRAP. N = 3 mice. Data are mean ± SEM in (b, c). Two-way ANOVA analysis followed by post hoc Sidak tests was used in (b, c).
