Extended Data Fig. 9: Lac-Phe enhances KATP channels in AgRP neurons of female mice.
From: Lac-Phe induces hypophagia by inhibiting AgRP neurons in mice
(a) Representative voltage-clamp traces for KATP currents in AgRP neurons of female mice at the baseline (black) or in response to 5 μM Lac-Phe (red) in the absence or presence of tolbutamide. (b) Quantifications of KATP currents in AgRP neurons in response to various treatments as described in (a). N = 6 or 8 neurons from 3 different mice. p < 0.0001 for vehicle vs Lac-Phe and Lac-Phe vs Lac-Phe+Tol. (c) Representative current-clamp traces showing effects of vehicle, Lac-Phe (5 μM), or Lac-Phe (5 μM) plus tolbutamide (100 μM) on AgRP neurons in the ARH of female mice. (d-e) Temporal changes in the resting membrane potential (d) and firing frequency (e) of AgRP neurons in the ARH in response to various treatments as described in (c). N = 6 neurons from 3 different female mice. p < 0.0001 for vehicle vs Lac-Phe and Lac-Phe vs Lac-Phe+Tol in both d and e. Data are mean ± SEM with individual data points in (b) and mean ± SEM in (d, e). Two-way ANOVA analysis followed by post hoc Sidak tests was used in (b, d, e). *, p < 0.05; **, p < 0.01; ****, p < 0.0001.
