Extended Data Fig. 1: Lac-Phe does not induce adverse effects.
From: Lac-Phe induces hypophagia by inhibiting AgRP neurons in mice
(a) Three-hour food intake in chow-fed female WT mice (2 months of age) receiving i.p. injections of vehicle or Lac-Phe (50 mg/kg). p = 0.016. (b) Three-hour food intake in HFD-fed female WT mice (3 months of age) receiving i.p. injections of vehicle or Lac-Phe (50 mg/kg). p = 0.0047. (c) Three-hour kaolin intake in male WT mice (3 months of age) receiving i.p. injections of vehicle or Lac-Phe (50 mg/kg). (d) Flavor preference ratio in male WT mice (3 months of age) receiving i.p. injections of vehicle or Lac-Phe (50 mg/kg). (e) Travel distance, velocity, number of entries into the center and time spent in the center during OFT in male WT mice (3 months of age) receiving i.p. injections of vehicle or Lac-Phe (50 mg/kg). (f) Travel distance, velocity, number of entries into the open arms and time spent in the open arms during OFT in male WT mice (3 months of age) receiving i.p. injections of vehicle or Lac-Phe (50 mg/kg). (g) Number of entries into the closed arms and time spent in the closed arms during OFT in male WT mice (3 months of age) receiving i.p. injections of vehicle or Lac-Phe (50 mg/kg). (h) Time spent in immobility in FST in male WT mice (3 months of age) receiving i.p. injections of vehicle or Lac-Phe (50 mg/kg). (i) Sucrose preference ratio in SPT in male WT mice (3 months of age) receiving i.p. injections of vehicle or Lac-Phe (50 mg/kg). N = 8 mice in each group for a-i.Data are mean ± SEM with individual data points in (a-i). Two-sided unpaired t-test was used in (a-i). *, p < 0.05; **, p < 0.01.
