Extended Data Fig. 4: i.p. injection of Lac-Phe increases its concentration in the ARH.
From: Lac-Phe induces hypophagia by inhibiting AgRP neurons in mice
(a-c) Body weight (a), body composition (b), and blood glucose (c) changes in chow- and HFD-fed male mice. N = 19 mice in each group. p < 0.0001 for b; p = 0.0086 for c. (d-e) Lac-Phe concentration in the serum (d) and ARH (e) of chow-fed male mice at 0, 5, 10, 30, 60 and 120 min after an i.p. injection of 50 mg/kg Lac-Phe. N = 4 mice for 0 and 5 min, N = 3 for 10, 30, 60, and 120 min. p = 0.0022 for 0 vs 5; p = 0.0005 for 0 vs 10; p = 0.015 for 0 vs 30 min in d; p = 0.0003 for 0 vs 5; p = 0.0011 for 0 vs 10 min in e. (f-g) Lac-Phe concentration in the serum (f) and ARH (g) of HFD-fed male mice at 0, 5, 10, 30, 60 and 120 min after an i.p. injection of 50 mg/kg Lac-Phe. N = 4 mice for 0, 5 and 10 min, N = 3 for 30, 60, and 120 min. p = 0.0085 for 0 vs 5; p = 0.0035 for 0 vs 10; p = 0.0059 for 0 vs 30, p = 0.0425 for 0 vs 60 min in f; p = 0.0009 for 0 vs 5; p = 0.0184 for 0 vs 10; p = 0.0293 for 0 vs 30, p = 0.0372 for 0 vs 60 min in g. (h-i) Serum (h) and ARH (i) Lac-Phe concentrations in chow and HFD-fed male mice at 0, 5, 10, 30, 60 and 120 min after an i.p. injection of 50 mg/kg Lac-Phe. Note that panels h-i were plotted using the same data from d-g. Data are mean ± SEM with individual data points in (b, d-g) and mean ± SEM in (a, c, h-i). One-way ANOVA analysis was used in (d-g); two-way ANOVA analysis was used in (a-c, h-i). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
