Extended Data Fig. 1: Acetate levels in HCC tissues and the flow cytometry gating strategy of TAMs.
a, b, The levels of acetate in HCC tumor tissues (a) and tumor-derived interstitial fluid samples (b) in comparison to the corresponding adjacent normal liver. Data are shown as mean ± SEM (n = 7 mice per group) (a), or mean ± SEM (n = 5 mice per group) (b). HCC tumor tissues and normal liver tissues were from Hepa1-6 orthotopic HCC mice model. C57BL/6 J mice were orthotopically injected with Hepa1-6-ZsGreen cells. 14 days later, the mice were sacrificed, and the tumor tissues were used for isolation of primary TAMs, TANs and NCCs by flow cytometry. The flow cytometry gating strategy is shown in (c). TAMs were depleted in Hepa1-6-ZsGreen orthotopic HCC mice model by intraperitoneal injection of clodronate liposomes every 2 days for three times. The depletion efficiencies were then determined by measuring the infiltration of CD45+Ly6G−F4/80+CD11bint cells by flow cytometry (d), and primary Hepa1-6-ZsGreen cells were isolated from tumor tissues by flow cytometry. The flow cytometry gating strategy is shown in (e). Representative images result from at least three independent experiments are shown. P values were determined by unpaired two-tailed Student’s t-tests (a, b).
