Extended Data Fig. 10: The protective effect of IPA against DSS colitis is dependent on CD4 + T cell TH1 and TH17 metabolic reprogramming without altering Treg phenotype in vivo.
(A) Table showing the clinical data of healthy controls and IBD patient cohort. (B) Schematic diagram showing the protocol of DSS colitis experiment treated with or without IPA. IPA (200 mg/kg) or corresponding vehicle control were administered to the mice daily by oral gavage. Created with BioRender.com. (C) Metabolic profile of colonic CD8 + T cell, B cell and γδ T cell of control (Con), DSS and DSS + IPA mice. n = 7 for Con, n = 9 for DSS, n = 6 for DSS + IPA. (D-E) Flow cytometry and its quantification of colonic CD4 + T cells stained intracellularly for FOXP3. n = 5 for Con, n = 6 for DSS and DSS + IPA groups. (F-G) Body weight curve and DAI scoring during the whole process of DSS model in Rag2-/- mice. n = 5 for Con, n = 6 for DSS and DSS + IPA groups. (H-J) Colon length, histology score and H & E staining of colon tissue at D7 in Con, DSS and DSS + IPA Rag2-/- mice. n = 5 for Con and DSS; n = 6 for DSS + IPA groups for colon length comparison. For H & E histology, n = 4 for Con and DSS; n = 3 for DSS + IPA groups were randomly selected. Scale bar: 200 μm. Panel (E), (H), (I) represent mean ± SEM analysed by one-way ANOVA with Tukey’s correction for multiple comparisons. Panel (C), (F), (G) represent mean ± SEM analysed by two-way ANOVA with Tukey’s correction for multiple comparisons.
