Fig. 6: IPA alters energy metabolism of CD4+ T cell towards mitochondria respiration in vivo. | Nature Metabolism

Fig. 6: IPA alters energy metabolism of CD4+ T cell towards mitochondria respiration in vivo.

From: Microbial metabolite indole-3-propionic acid drives mitochondrial respiration in CD4+ T cells to confer protection against intestinal inflammation

Fig. 6: IPA alters energy metabolism of CD4+ T cell towards mitochondria respiration in vivo.

a, Schematic diagram showing the protocol of ABX treatment and SCENITH of small intestine and colon LP. IPA (200 mg kg−1) or corresponding vehicle control were administered to mice daily by oral gavage. Created with BioRender.com. b, Total faecal bacteria amount of WT, ABX and ABX + IPA group. n = 5. c,d, The proportion of each cell population including CD4+, CD8+, DN and CD4+CD8+ double positive cell subsets under the TCRαβ+ cell gate in colonic LP of WT, ABX and ABX + IPA mice. n = 7 for WT and ABX + IPA, n = 9 for ABX. (e) ATP synthesis in WT, ABX and ABX + IPA group of CD4+ and CD8+ T cell subsets in colon LP. n = 16 for WT, n = 15 for ABX, n = 12 for ABX + IPA. f, Metabolic profile of CD4+ T cells of colonic LP in WT, ABX and ABX + IPA group. n = 13 for WT, n = 12 for ABX and ABX + IPA groups. g, Metabolic profile of CD8+ T cells of colonic LP in WT, ABX and ABX + IPA group. n = 13 for WT, n = 12 for ABX and ABX + IPA groups. Data represent mean ± s.e.m. analysed by one-way ANOVA with Tukey’s correction for multiple comparison (b,d,e) and mean ± s.e.m. analysed by two-way ANOVA with Tukey’s correction for multiple comparisons (f,g).

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