Fig. 8: Anti-inflammatory effect of IPA in colitis model in vivo are mediated by CD4+ T cells.
a, Schematic diagram showing the protocol of adoptive transfer model. Created with BioRender.com. b,c, Body weight curve (c) and DAI scoring (d) of Rag2−/− recipient mice that received either no-IPA-treated CD4+ T cells or IPA-treated CD4+ T cells during the whole adoptive transferred process. n = 8 for no-IPA and n = 6 for IPA group. d–f, H&E staining (d), colon length (e) and histology score (f) of colon tissue at D13 in no-IPA CD4+ T→Rag2 and IPA CD4+ T→Rag2 mice. n = 8 for no-IPA and n = 6 for IPA group for colon length comparison. For H&E histology, n = 3 for no-IPA and n = 4 for IPA group were randomly selected. Scale bars, 200 μm. g, Schematic diagram showing the protocol of adoptive transfer model. Created with BioRender.com. p.o., oral administration. h,i, Body weight curve (h) and DAI scoring (i) of Rag2−/− recipient mice that received either ABX CD4+ T cells or ABX + IPA CD4+ T cells during the whole adoptive transferred process. n = 9 for ABX and n = 7 for ABX + IPA group. j–l, H&E staining (j), colon length (k) and histology score (l) of colon tissue at D12 in ABX CD4+ T→Rag2 and ABX + IPA CD4+ T→Rag2 mice. n = 9 for ABX and n = 7 for ABX + IPA group for colon length comparison. For H&E histology, five samples were randomly selected. Scale bars, 200 μm. All data (except a,d,g,j) represent mean ± s.e.m. analysed by two-tailed unpaired Student’s t-test.
