Extended Data Fig. 8: Effects of rolipram on mitochondrial translation-related gene expression, on phospho-AMPK, and on endurance performance in C26-bearing mice. | Nature Metabolism

Extended Data Fig. 8: Effects of rolipram on mitochondrial translation-related gene expression, on phospho-AMPK, and on endurance performance in C26-bearing mice.

From: Impaired cAMP–PKA–CREB1 signalling drives mitochondrial dysfunction in skeletal muscle during cancer cachexia

Extended Data Fig. 8: Effects of rolipram on mitochondrial translation-related gene expression, on phospho-AMPK, and on endurance performance in C26-bearing mice.

a, Heatmap of normalized RPKM of rolipram-rescued DEGs selected from MitoXplorer gene lists “Translation” in vehicle- or rolipram-treated mice (C26 veh and C26 Rol) as compared to controls (ctr). n = 7 ctr, n = 7 C26 veh, and n = 5 C26 Rol mice. b,c, Representative image (b) and densitometry analysis (c) of p-AMPKT172, total AMPK, and vinculin of ctr, C26 veh, and C26 Rol mice. Densitometry of p-AMPKT172 was normalized over vinculin. n = 3 mice for each group. 1-way ANOVA. d, Endurance performance measured through Treadmill test; distance run in 60 min by ctr, C26 veh, and C26 Rol mice. n = 8 ctr, n = 7 C26 veh, n = 6 C26 Rol mice; 1-way ANOVA. Data are presented as mean values ± SEM in c and d, significant P values are annotated in the graphs.

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