Table 1 The GSM of ChREBPα directly binds G3P

From: Glycerol-3-phosphate activates ChREBP, FGF21 transcription and lipogenesis in citrin deficiency

Ligand

Kd ± s.d. (μM)

G3P

17.0 ± 1.1

G6P

64.3 ± 13.5

F6P

131 ± 43

X5P

164 ± 16

GA3P

221 ± 62

F1,6BP

250 ± 37

Glucose

270 ± 54

DHAP

453 ± 31

  1. Ligand affinities were determined in triplicate by isothermal titration calorimetry using a construct of purified mouse ChREBP GSM in which residues 43–307 were expressed carboxyl to MetHis6. The data show that G3P is the highest-affinity ligand for the GSM. We suggest that hereafter the GSM be considered an abbreviation for G3P-sensing module.
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