Extended Data Fig. 3: AFABP underlies the obesity-induced hindrance of intestinal epithelial regeneration.

a-d, Quantification of the ratio of esg-GFP⁺ cells to DAPI⁺ cells (a), Dl⁺ cells to DAPI⁺ cells (b), the number of pH3⁺ cells per gut (c), and NRE-lacZ⁺ cells to esg-GFP⁺ cells (d). n = 15 midguts each group (c). LUC^RNAi as control. e-g, Quantification of the ratio of esg-GFP⁺ cells to DAPI⁺ cells (e), Dl⁺ cells to DAPI⁺ cells (f), and NRE-lacZ⁺ cells to esg-GFP⁺ cells (g) n = 15 midguts each group. LUC^RNAi as control. h, mRNA expression of EC maturation-associated genes. n = 3 independent experiments. i-l, Quantification of the ratio of NRE⁺ cells to esg-GFP⁺ cells (i), esg-GFP⁺ cells to DAPI⁺ cells (j), Dl⁺ cells to DAPI⁺ cells (k), and the number of pH3⁺ cells per gut (l) n = 15 midguts each group. LUC^RNAi + NCD as control. m, Relative mRNA expression of COPI genes. n = 3 independent experiments. LUC^RNAi as control. n, A volcano plot illustrating the differential expression of genes from mesenteric adipose tissue of obesity and NW mice. o, A bar chart depicting upregulated genes with red bars and downregulated genes with green bars of obesity and NW mice. p, Relative mRNA expression of dFabp genes. n = 3 independent experiments. LUC^RNAi as control. q-t, Quantification of the ratio of esg-GFP⁺ cells to DAPI⁺ cells (q), Dl⁺ cells to DAPI⁺ cells (r), NRE-lacZ⁺ cells to esg-GFP⁺ cells (s) (n = 11-15 midguts each group) and the number of pH3⁺ cells per gut (n = 15 midguts) (t). u, The AlphaFold structural prediction for both the fruit fly dFABP protein and its human counterpart, AFABP. Error bars indicate mean +/- SDs, ns (not statistically significant) signifies p > 0.05. LUC^RNAi as control. Experiments were performed three times. One-way ANOVA with Dunnett multiple comparison test for (a-l, p). Unpaired two-tailed Student’s t-tests for (m, q-t). Additional data are provided in Tables S1, S,4 and S6.

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