Extended Data Fig. 10: Enhanced Peroxisomal Function Promotes Intestinal Regeneration in AFABP-Overexpressing Drosophila.

a, Representative images of midguts with NRE-lacZ (red) and esg-GFP (green) staining from 3 independent experiments. b, Quantification of the ratio of NRE-lacZ⁺ cells to esg-GFP⁺ cells (n = 14 midguts for each group). c-g, Quantification of the ratio of esg-GFP⁺ cells to DAPI⁺ cells (c and e), Dl⁺ cells to DAPI⁺ cells (d and f), and NRE-lacZ⁺ cells to esg-GFP⁺ cells (g) (n n=numbers of midguts for each group as indicated in figures). h-m, Quantification of deposits (h and m), the ratio of homeostasis state (i and k), and the ratio of Smurf (+) files (j and l). (h, n = 16, 20 fields of view for each group), (i, n = 6 midguts respectively), (j, n = 5, 7 independent experiments), (k, n = 7, 5, 8, 5 independent experiments), (l, n = 5, 4, 5, 6 independent experiments), (m, n = 25, 14, 17, 14 fields of view for each group). n-p, Quantification of the ratio of esg-GFP⁺ cells to DAPI⁺ cells (n), NRE-lacZ⁺ cells to esg-GFP⁺ cells (o) and Dl⁺ cells to DAPI⁺ cells (p) (n = 20, 26, 23 midguts for each group respectively). NaPB, pioglitazone (two agents that enhance peroxisome proliferation through PEX11 stimulation) were used. q-s, Quantification of midgut length (n = 24, 26, 23 midguts respetively) (q), deposits (n = 20, 22, 21fields of view) (r), and the ratio of Smurf (+) files (n = 6 independent experiments) (s). t, Relative mRNA expression of pex11ab genes from indicated genotypes (n = 3 independent experiments). u, Relative mRNA expression of Eip75b genes from indicated genotypes (n = 3 independent experiments). v, w, Western blot representative images (v) and quantification (w) of PPARα after injury in mice, n = 3 independent experiments. x-aa, Relative mRNA expression of Pparα (x) and its target genes (y–aa) after injury in mice, n = 3 mice. Nuclei stained with DAPI are shown in blue. Scale bars represent 20 μm (a). Error bars indicate mean +/- SDs. Unpaired two-tailed Student’s t-tests for (h-j, t, u), one-way ANOVA with Dunnett multiple comparison test for other results. Significance: ns signifies p > 0.05. Additional data are provided in Tables S4 and S6.

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