Extended Data Fig. 5: DREADD ligands reduce RER but do not affect thermogenesis or glucose tolerance.
From: Distinct sympathetic projections to brown fat regulate thermogenesis and glucose tolerance
a, Schematic illustration of the random crossover experimental design to assess the independent impact of DCZ and CNO versus saline treatment in awake WT mice (data shown in b-f). b, Effects of CNO, DCZ, or saline delivered immediately before a glucose gavage on circulating glucose levels. Data presented as the mean ± s.e.m. (n = 7 mice). c, Within-subject effects (DCZ minus saline and CNO minus saline) on blood glucose levels from the same mice shown in b. d, Average traces of energy expenditure (kcal/h). Solid lines represent means, shaded areas represent ± s.e.m. (n = 7 mice). e, f, Changes in energy expenditure (e) and respiratory exchange ratio (RER, f) following saline, DCZ, or CNO injection (n = 7 mice). Dots represent average values, lines represent individual mice. f, Repeated measure two-way ANOVA (sex and treatment, P = 0.418 for sex and P = 0.043 for treatment), *P < 0.05 after Dunnett’s post-hoc test with saline as reference.
