Extended Data Fig. 4: Effects of glycosylation inhibitors on LIF release and cellular protein content.
A. Glycosylation as fold change vs. Glc 25 mM in A549. Densitometric quantification of the concanavalin A WB in Fig. 4b (n = 4). B. LIF secretion (non-glycosylated) from A549 treated for 24 h (n = 3). C-E. A549 cells were treated for 24 h with indicated drugs or DMSO (labeled as “D”). LIF secretion was measured at 24 h using R&D hLIF Quantikine ELISA (total LIF) (C, D), and protein content is indicated in panel E). n indicated in figure. F-H. SW900 and H1299 cells were incubated for 24 h with indicated drugs or DMSO (labeled as “D”). Total LIF release is shown in F and G. Protein content is shown in H. n indicated in figure. Graphs show average values and individual replicates, and error bars represent the standard error of the mean (SEM); statistical significance was determined by one-way ANOVA with multiple comparisons test.
