Extended Data Fig. 5: Metabolic features of CrGPDH expression in 786-O cells.
From: A genetically encoded bifunctional enzyme mitigates redox imbalance and lipotoxicity
Glycerol (a), lactate (b), pyruvate (c) and the lactate/pyruvate ratio (d) measured by GC-MS in media which was incubated for 24 hours (spent media) with 786-O cells expressing Luciferase (LUC) or CrGPDH. Glucose consumption (e), lactate production (f) and glutamine consumption (g) measured by the YSI 2900 Biochemistry Analyzer in media which was incubated for 24 hours (spent media) with 786-O cells expressing Luciferase (LUC) or CrGPDH. Targeted metabolomics of 786-O cells expressing CrGPDH relative to LUC control (h). Significantly increased metabolites (p value cutoff < 0.05, fold change >1) are highlighted in red, and significantly decreased metabolites (p value cutoff < 0.05, fold change < -1) are highlighted in dark blue, while gray dots represent metabolites without significant changes. Experiments in (a-g) were performed in pyruvate-free RPMI+dFBS containing 5 mM glucose; in (h) were performed in pyruvate-free RPMI+dFBS containing 25 mM glucose. UDP-GlcA: UDP-glucuronic acid. Values are mean ± s.d.; n = 6, 6 in (a-g) biologically independent samples. The statistical significance indicated for (a-g) represents a two-tailed unpaired t test. NS, no significant difference.
