Extended Data Table 1 Steady-state kinetic parameters of CrGPDH

From: A genetically encoded bifunctional enzyme mitigates redox imbalance and lipotoxicity

  1. aFor all substrate pairs, activity was measured using 87CrGPDH705 variant by following the absorbance at 340 nm (the dehydrogenase reaction). When NADH was varying substrate, DHAP was fixed at 5 mM; when DHAP was varying substrate, NADH was fixed at 500 µM. bNo activity was detected with NADPH. Details of enzymatic assays are described under Methods.
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