Fig. 4: Letm1 KD modulates metabolism and improves LTM formation in rodents.
From: Mitochondrial Ca2+ efflux controls neuronal metabolism and long-term memory across species
a,b, Visualization of the hippocampal brain region injected with the corresponding viral constructs, showing mRFP1 fluorescence in red and DAPI in blue. Scale bars, 500 μm. c, Left, representative western blot showing levels of Letm1 and β-actin proteins in lysates from dissected hippocampi of control and Letm1 KD mice. Right, quantification of Letm1 expression relative to controls. P value was determined using a two-tailed Wilcoxon signed-rank test (**P = 0.0078; n = 8; W = −36). d, Left, representative western blot showing levels of phosphorylated PDH (pPDH-S293) and total PDH in lysates from dissected hippocampi of control and Letm1 KD mice. Right, quantification of pPDH-S293 intensity relative to total PDH. P value was determined using a two-tailed Wilcoxon signed-rank test (**P = 0.0078; n = 8; W = 36). e, Schema showing the aversive olfactory conditioning paradigm in mice for MTM and LTM. f, MTM in control and Letm1 KD mice shows no difference between conditions. P value for MTM was determined using a two-tailed Mann–Whitney test (NS, P = 0.9395; n = 8 for control and n = 8 for Letm1 KD; U = 31). g, LTM in control and Letm1 KD mice shows that memory is only preserved in mice expressing Letm1 KD in the hippocampus. P value for LTM was determined using a two-tailed Mann–Whitney test (*P = 0.0303; n = 8 for control and n = 8 for Letm1 KD; U = 11.5). Data are represented as the mean ± s.e.m. See also Extended Data Fig. 7.
