Extended Data Fig. 9: Iron chelation and anti-lipid peroxidation interventions preserve NE integrity and extend lifespan.
a, Representative images of intestinal NE morphology in lmn-1 RNAi animals grown on OP50 bacteria with or without VE (5 mM) or fer-2 (2 mM) supplementation. b, Quantification of the intestinal nuclei is based on their morphology, as depicted in (a). c, Lifespan analysis of N2 animals grown on EV or cco-1 RNAi bacteria with or without NAC (8 mM) supplementation. d, Lifespan analysis of N2 animals grown on OP50 E.coli bacteria with or without fer-2 (2 mM or 5 mM) supplementation. e, Lifespan analysis of N2 animals grown with or without FINO2 (2 mM) supplementation. f, Lifespan analysis of N2 animals grown on EV bacteria with or without EPA (C22:5, 2 or 5 mM) supplementation. g, Lifespan analysis of N2 animals grown on EV or cco-1 RNAi bacteria with or without Fer-2 or FINO2 (Fer-2 5 mM, FINO2 2 mM) supplementation. h, Lifespan analysis of N2 animals grown on EV or cco-1 RNAi bacteria with or without 2,2’-bipyridyl (bpy, 10 μM) supplementation. i, Lifespan analysis of N2 animals grown on EV or cco-1 RNAi bacteria with or without 2 2’-bipyridyl (bpy, 50 μM) supplementation. 100 nuclei from more than 20 animals were examined for each group (a); the exact number of NE in each class was provided as source data. 3 independent experiments were analyzed in each condition (b). Scale bars, 10 μm (a). Data are mean ± s.e.m. P values were derived from two-sided Pearson’s chi-square tests (b); lifespan data were analyzed with Kaplan-Meier (c,d,e,f,g,h,i). Source numerical data are available in the source data.
