Extended Data Fig. 3: SLC6A6 is required for mitochondrial translation and tRNA modifications.
From: SLC6A6 imports taurine into mitochondria to sustain mitochondrial translation and tumour growth
a, Immunoblot analysis of puromycin incorporation into mitochondrial encoded proteins from In Organello Translation assay. TOM20 serves as loading control. b, Secondary structures of hmtRNATrp with indicated tm5U modification. Arrows indicate specific RNase T1 cleavage sites generating U34‑containing fragments. c, Intensity fractions (%) of U34-containing fragments with different modification states in the indicated A549 cells. Sequences are annotated with their mass-to-charge ratio (m/z), charge state, and relative modification frequencies. Experiments were repeated three times independently, with similar results (a).
