Extended Data Fig. 4: SLC6A6 deficiency alters glucose and glutamine metabolism in cancer cells.
From: SLC6A6 imports taurine into mitochondria to sustain mitochondrial translation and tumour growth
a, A scheme of [U-13C] glucose isotope labeling and flux analysis. b, [U-13C] glucose-derived citrate M + 2 labeling percentage (% of total pool) of A549 and Huh7 cells in control and SLC6A6 knockdown groups. n = 3 biological replicates. c, A scheme of [U-13C] glutamine isotope labeling and flux analysis. d, e, [U-13C] glutamine-derived citrate M + 4 (d) and M + 5 (e) labeling percentage (% of total pool) of A549 and Huh7 cells in control and SLC6A6 knockdown groups. n = 3 biological replicates. f, [U-13C] glutamine-derived Malate M + 3 and M + 4 labeling percentage (% of total pool) of A549 and Huh7 cells in control and SLC6A6 knockdown groups. n = 3 biological replicates. All statistical graphs show the mean ± s.e.m. P values were calculated using two-tailed Student’s t-test (b) and one-way ANOVA (d-f). Experiments were repeated three times independently, with similar results (b, d-f).
