Extended Data Fig. 8: NFAT5 is required for cancer cell growth and mitochondrial function.
From: SLC6A6 imports taurine into mitochondria to sustain mitochondrial translation and tumour growth
a, Kaplan–Meier overall survival plots stratified by SLC6A6 high (red) or low (blue) mRNA levels from TCGA database. b, Kaplan–Meier overall survival plots stratified by NFAT5 high (red) or low (blue) mRNA levels from TCGA database. A log-rank Mantel–Cox test was performed between the groups of each plot (a, b). c, Positive correlation between SLC6A6 and NFAT5 mRNA levels from pan-cancer analysis. d, e, Representative crystal violet staining (d) and quantification (e) of clonal formation from shCtrl and shNFAT5 cancr cells. n = 4 biological replicates (e). f, Quantification of shCtrl and shNFAT5 Huh7 and H1299 cell death (% trypan blue+) in galactose-containing medium (10 mM) for 48 h. n = 4 biological replicates. g, Relative taurine abundance in shCtrl and shNFAT5 A549 and Huh7 cells. n = 3 biological replicates. Data presented as mean ± s.e.m. of three independent experiments; statistical significance was determined by one-way ANOVA (e-g). Experiments were repeated three times independently, with similar results (d-g).
