Extended Data Fig. 1: Exogenous taurine is dispensable for liver and lung cancer cell growth.
From: SLC6A6 imports taurine into mitochondria to sustain mitochondrial translation and tumour growth
a, Relative growth of Hepa1-6 and HepaMP9-1 cells in D10 and D-N2B27 medium for 72 h. n = 4 biological replicates. b, Relative 293 T cell growth in D10 and D-N2B27 medium. c, d, Relative growth of of 293 T (c) and IMR90 (d) cells in DMEM containing indicated supplements for 72 h. n = 4 biological replicates. e, Relative growth of different cancer cells cultured in D10 or D-dFBS medium supplemented with indicated concentrations of taurine. n = 3 biological replicates. f, g, Representative crystal violet staining (f) and quantification (g) of clones from cancer cells cultured in D10 or D-dFBS medium supplemented with indicated concentrations of taurine. n = 3 biological replicates (g). h, Immunoblot analysis of taurine biosynthetic enzymes in different cell lines. HSP90 serves as loading control. All statistical graphs show the mean ± s.e.m. P values were calculated using a two-tailed Student’s t-test (a-c) or one-way ANOVA (d, e, g). Experiments were repeated at least three times independently, with similar results (a-h).
