Extended Data Fig. 2: SLC6A6 is required for liver and lung cancer cell growth.
From: SLC6A6 imports taurine into mitochondria to sustain mitochondrial translation and tumour growth
a, qPCR analysis of SLC6A6 mRNA levels from control (shCtrl) and SLC6A6 (sh6A6)/Slc6a6 (sh6a6) knockdown cancer cells. Representative results from three independent experiments are shown. b, c, Representative crystal violet staining (b) and quantification (c) of clonal formation from shCtrl and sh6A6 cancer cells. n = 4 biological replicates (c). d, Representative TMRE staining of shCtrl and sh6A6 cancer cells. Scale bar: 10 µm. e, Immunoblots of indicated mitochondrial proteins from shCtrl and sh6A6 cancer cells cultured in D-N2B27 medium. HSP90 serves as loading control. f, Relative growth of indicated groups of cancer cells cultured in D10 or D-dFBS medium. n = 3 biological replicates. g, Immunoblots of mitochondrial proteins from indicated groups cells cultured in D10 medium. HSP90 serves as loading control. All statistical graphs show the mean ± s.e.m. P values were calculated using one-way ANOVA (a, c, f). Experiments were repeated at least three times independently, with similar results (a-g).
