Extended Data Fig. 5: Proteomics reveals altered signaling pathways after Ecm1 knockdown in fibrotic kidneys after IRI. | Nature Metabolism

Extended Data Fig. 5: Proteomics reveals altered signaling pathways after Ecm1 knockdown in fibrotic kidneys after IRI.

From: Early-activated extracellular matrix proteins shape the metabolic and spatial dynamics of the kidney fibrotic microenvironment

Extended Data Fig. 5: Proteomics reveals altered signaling pathways after Ecm1 knockdown in fibrotic kidneys after IRI.The alternative text for this image may have been generated using AI.

(a) Quantitative data for the protein expression of integrin (ITG) A2, B1, AV, RhoC, RhoA, and CDC42 in AAV9-Scramble (Sc) and AAV9-ShEcm1 kidneys after unilateral IRI combined with nephrectomy (n = 6). (b, c) Quantitative data for the protein expression of ITGA2, ITGB1, and RhoC in Col1α2-Ecm1+/+ and Col1α2-Ecm1-/- (b, n = 6) or Pdgfrb-Ecm1+/+ and Pdgfrb-Ecm1-/- (c, n = 5) kidneys after unilateral IRI. (d) Structural representation of protein-protein interactions. The image shows the structure of ECM1 (purple), integrin β1 (blue), and integrin α2 (green). Key amino acid residues involved in hydrogen bonding and electrostatic interactions are labeled and depicted as sticks. Dashed lines indicate potential hydrogen bonds or salt bridges stabilizing the interaction interface. Data are presented as mean ± s.e.m. Differences between groups were analyzed using two-sided unpaired t tests.

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