Extended Data Fig. 6: Phosphoproteomics reveals energy metabolism changes are major events in fibrotic kidneys after knockdown of Ecm1. | Nature Metabolism

Extended Data Fig. 6: Phosphoproteomics reveals energy metabolism changes are major events in fibrotic kidneys after knockdown of Ecm1.

From: Early-activated extracellular matrix proteins shape the metabolic and spatial dynamics of the kidney fibrotic microenvironment

Extended Data Fig. 6: Phosphoproteomics reveals energy metabolism changes are major events in fibrotic kidneys after knockdown of Ecm1.The alternative text for this image may have been generated using AI.

(a) Poylogenetic tree of all protein kinases families detected in proteomics study. (b) Selected pathways of GO analysis for enriched up- and down-regulated phosphoproteins in the fibrotic kidneys after knockdown of Ecm1. (c) Quantitative data for the protein expression of LATS1, YAP, and TAZ in AAV9-Scramble (Sc) and AAV9-ShEcm1 kidneys after unilateral IRI combined with nephrectomy (n = 6). (d) Bioinformatics analysis showing TEAD4 is predicted to repress Pgc1a. (e) Human TEAD 4 motif. (f) Yap Dsi (rat) was transfected into NRK-52E cells. (g, h) Quantitative data for the protein expression of YAP and TAZ in Col1α2-Ecm1+/+ and Col1α2-Ecm1-/- (g, n = 6) or Pdgfrb-Ecm1+/+ and Pdgfrb-Ecm1-/- (h, n = 5) kidneys after unilateral IRI. Data are presented as mean ± s.e.m. Differences between groups were analyzed using two-sided unpaired t tests.

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