Extended Data Fig. 4: Additional metabolic assessments for male Rab27a KD model.
(a) Male Rab27a KD mice and matched Con mice maintained on normal chow were assessed for energy expenditure (EE) without fasting in metabolic cages. EE values were shown per animal without body weight adjustment. (b–d) After the development of feeding disorder, weight gain, and hyperglycemia under chow feeding, male Rab27a KD subjected to a 10-day rescue experiment in which animals received daily intranasal administration of tanycytic EVs (1 µg) vs. vehicle. Same treatment also applied to Con group for additional information. These animals during the treatment were monitored for food intake (b) and body weight (c) and followed by fasting blood glucose (d) at the end of this treatment. Statistics: two-tailed unpaired t-test (a); two-way repeated measures ANOVA/Tukey post-hoc (b, c), and one-way ANOVA/Tukey post-hoc (d). n = 6 mice per group (a–d); data reflect mean ± SEM.
