Extended Data Fig. 5: Composition assessment of Rab27a KD EVs versus control EVs.
EVs from tanycytes with Rab27a KD vs. matched control (Con) were purified and analyzed by Western blot (a) and proteomics (b). Proteomic datasets were analyzed for protein-level differences based on uncorrected p values and corrected false discovery rate (FDR) q values. The threshold for differential expression levels was set at a 2-fold change based on standard variation criteria. Statistics: two-tailed unpaired t-test; n = 3 biological samples per group.
