Extended Data Fig. 9: Proteomics of EVs deficient of ppIns or Rictor versus control.
EVs from tanycytes with ppIns KD samples vs. matched shRNA-based Control (Con) samples, or Rictor KD samples vs. matched gRNA-based Con samples (the same control in Extended Data 5), were subjected to proteomics (a, b) and measurement of total small RNAs (c). Statistical analysis of protein-level differences was then analyzed based on uncorrected p values and corrected FDR q values (a, b). The threshold for differential expression levels was set at a 2-fold change based on standard variation criteria. Statistics: two-tailed unpaired t-test; n = 3 biological samples per group (a–c); data in bar graphs reflect mean ± SEM.
