Extended Data Fig. 5: Deletion of epithelial ENO2 restores the function of tumor-infiltrating CD8⁺ T cell in Trip6-deficient KRAS wild-type mice.

a, A schematic illustrating the generation of Trip6^−/−;Eno2^ΔIEC mice (top) and the experimental protocol (bottom). b-d, Representative images of colons (b, left) and H&E-stained sections (b, right), alongside quantifications of colon tumor numbers (c) and tumor size (d) (Trip6^+/+;Eno2^fl/fl: n = 12 mice, Trip6^−/−;Eno2^fl/fl: n = 23 mice, Trip6^+/+;Eno2^ΔIEC: n = 16 mice, Trip6^−/−;Eno2^ΔIEC: n = 23 mice). e, ELISA showing lactate levels in colon tumors from the indicated mice (Trip6^+/+;Eno2^fl/fl: n = 8 mice, Trip6^−/−;Eno2^fl/fl: n = 7 mice, Trip6^+/+;Eno2^ΔIEC: n = 9 mice, Trip6^−/−;Eno2^ΔIEC: n = 8 mice). f-h, Flow cytometry analysis of colon tumors showing the proportions of CD8⁺ T cells (f), Ki67⁺CD8⁺ T cells (g), TNF⁺CD8⁺ and GzmB⁺CD8⁺ T cells (h) in the indicated mouse groups (n = 5 mice per group). The data are representative of three independent experiments and presented as mean ± s.d. P values were determined using one-way ANOVA followed by Tukey’s test (c-h).

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