Extended Data Fig. 5: Irisin directly induces IL-33 expression in stromal cells from eWAT but not in those from iWAT or BAT.

(A) Representative gating strategy for analyzing IL-33⁺ mSCs. (B) Summary data for numbers of IL-33⁺ mSCs in eWATs from the male mice in Extended Data Fig. 3F (n = 4, 4, 8, 8). (C) Summary data for numbers of IL-33⁺ mSCs in iWATs and BATs from the mice in Extended Data Fig. 3F and Fig. 2 (left, n = 4, 4, 8, 8; right, n = 3, 3, 8, 8). (D) ELISA measurements of IL-33 concentration in iWAT and BAT lysates from a subset of mice in Fig. 3c (n = 3, 3, 3, 3). One-way ANOVA was used for calculating p values (* p = 0.0159, *** p = 0.0002, and ** p = 0.0050). (E) Summary data for numbers of ILC2s in eWAT, iWAT and BAT from the mice in Extended Data Fig. 4 (top, n = 4, 4, 8, 8; bottom, n = 3, 3, 8, 8). (F) Freshly isolated IL-33 (EGFP)⁻ mSCs from 12 wk-old male Il33.Egfp mice were treated with PBS or 10 nM/Hsp90α irisin for 3 days, followed by flow-cytometric analysis. Left: experimental scheme; right: summary data for numbers and fractions of IL-33 (EGFP)⁺ mSCs. n = 3. Abbreviation: mSCs, mesenchymal stromal cells; ILC2s, type 2 innate lymphoid cells; other abbreviations as per Figs. 1 and 2. Mean ± SD.* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant. Significant but irrelevant p values are not indicated. Unless specifically mentioned, experiments were repeated twice with similar results. Mouse illustration in f created in BioRender; A, M. https://biorender.com/5v6fzeu (2026).

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