Extended Data Fig. 7: Irisin promotes IL-33 response in iWAT.
From: Irisin ameliorates obesity and insulin resistance via adipose tissue IL-33 and regulatory T cells
(A) Summary data of the number of total CD4+ T cells, as well as the percentages of Treg cells and ST2+ Treg cells in the spleen of the indicated mice from the flow-cytometric analysis. (B–E) 8 wk-old male Fndc5+/+ or Fndc5−/− littermates were treated as per Fig. 4c. (B) Summary data for the number of total Treg cells and the fraction of ST2+ Treg cells in iWAT. n = 3, 4, 3, 3. One-way ANOVA was used for calculating p values (* p = 0.0269). (C, D) Representative gating strategy for analyzing ILC2s (C) and Eosinophils (D). (E, F) Summary data for numbers of ST2+Foxp3−CD4+ cells, ILC2s, and Eosinophils in eWAT (n = 9, 9, 6, 8) (E) and iWAT (n = 3, 4, 3, 3) (F). Brown-Forsythe and Welch ANOVA (E) or one-way ANOVA (F) was used for calculating p values (E: * p = 0.0238, ** p = 0.0011, * p = 0.0426, **** p < 0.0001, and ** p = 0.0011; F: * p = 0.0333, * p = 0.0293, and * p = 0.0161). (G) ELISA measurements of irisin plasma concentrations in male Fndc5+/+ and Fndc5−/− mice fed a HFD for 17 weeks after AAV injection; n = 10, 11, 13, 13. One-way ANOVA was used for calculating p values (*** p = 0.0001, and *** p = 0.0006). (H) Pearson correlation analysis of area under ITT curves and body weight of male Fndc5−/− mice fed a HFD for 17 weeks after AAV injection; n = 16. Abbreviations as per Figs. 4 and 5. Mean ± SD.* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant. Significant but irrelevant p values are not indicated. Experiments in B-F were repeated three times with similar results, and the rest of the experiments were repeated twice with similar results.
