Extended Data Fig. 8: IL-33/ST2 axis is not required for regulating food intake, physical activities or thermogenic gene expression in eWAT of HFD-treated mice.
From: Irisin ameliorates obesity and insulin resistance via adipose tissue IL-33 and regulatory T cells
(A, B) Purification of recombinant IL-33trap (A) and measurement of endotoxin content (B). (C) ELISA measurements of irisin plasma concentrations in male mice fed a HFD for 17 weeks after AAV injection w and w/o chronic IL-33trap treatment (n = 13, 13, 13, 13). One-way ANOVA was used for calculating p values (**** p < 0.0001, * p = 0.0255, and **** p < 0.0001). (D) Body weight measurements of male mice fed a HFD for 17 weeks after AAV injection w and w/o chronic IL-33trap treatment (mice used for CLAMS); n = 6, 6, 6, 6. One-way ANOVA was used for calculating p values (** p = 0.0049, ** p = 0.0064, and ** p < 0.0033). (E–G) Accumulated food intake (E), locomotive activity (F) and total distance the animal traveled (G) measured in CLAMS cages over one week. n = 4, 4, 4, 4. The last 4 days of measurement were shown on the left and the averaged values in the light and dark periods were summarized on the right. Two-way ANOVA was used for calculating p values. (H–J) Body weight (H), eWAT weight (I) and iWAT weight (J) measurements (n = 11, 10, 4, 7). One-way ANOVA was used for generating p values (H: **** p < 0.0001, ns p = 0.9928, and * p = 0.0330; J: **** p < 0.0001, *** p = 0.0002, and ns p = 0.4604). (K) ELISA measurements of irisin plasma concentrations in mice; n = 12, 7. Unpaired two-sided T-test was used for calculating p values (ns p = 0.2468). (L) Related to Fig. 7f. RT-qPCR measuring mRNAs associated with thermogenesis and adipogenesis in eWAT of ST2 Treg null mice (Il1rl1Δtreg) and their littermates (Il1rl1wt) received AAV-irisin or -GFP and fed a HFD for 17 weeks (n = 11, 10, 4 7). Two-way ANOVA was used for calculating p values (**** p < 0.0001, **** p < 0.0001, and **** p < 0.0001). Abbreviations as per Fig. 1. Mean ± SD.* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant. Significant but irrelevant p values are not indicated. Unless specifically mentioned, experiments were repeated twice with similar results.
