Extended Data Fig. 3: Characterization of spontaneously immortalized bovine fibroblasts. | Nature Food

Extended Data Fig. 3: Characterization of spontaneously immortalized bovine fibroblasts.

From: Spontaneous immortalization of bovine fibroblasts following long-term expansion offers a non-transformed cell source for cultivated beef

Extended Data Fig. 3

(a) Doubling time of bovine fibroblasts in extended in vitro cultures. Spontaneously immortalized lines are referred to as HUN-BF-15 and HUN-BF-13 derived from Holstein and Simmental cows, respectively. (b) Phase images of primary fibroblasts and emerging fibroblast colonies. Black arrows indicate senescent cells, red arrow indicates edge of emerging colony of immortalized fibroblasts. Scale bar 200 µm. (c) Accumulative population doublings of immortalized lines HUN-BF-15 and HUN-BF-13 as a function of passage number. (d) Top 24 terms appearing in the enrichment analysis of overlapping differentially expressed genes between Sn-BF-15 and HUN-BF-15, and Sn-BF-13 and HUN-BF-13, respectively (n=3).

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