Extended Data Table 1 Primer sequences for species validation by PCR

From: Spontaneous immortalization of bovine fibroblasts following long-term expansion offers a non-transformed cell source for cultivated beef

Gene

Forward Primer

Reverse Primer

MT-CYTB (Bos taurus)

5’-CAATAACTCAACACAGAATTTGC-3’

5’-CGTGATCTAATGGTAAGGAATA-3’

MT-CYTB (Ovis aries)

5’-TACCAACCTCCTTTCAGCAATT-3’

5’-TGTCCTCCAATTCATGTGAGTGT-3’

Mt-CytB (Gallus gallus)

5’-AGCAATTCCCTACATTGGACACA-3’

5’-GATGATAGTAATACCTGCGATTG-3’

MT-CYTB (Homo sapiens)

5’-ACTTGTCCAATGATGGTAAAGG-3’

5’-TAGCAATAATCCCCATCCTCCAT-3’

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