Extended Data Fig. 6: scRNA-seq reveals heterogeneity and activation of CD8+ T lymphocytes in the aged CNS.
a, UMAP visualization of CD8+ T cells freshly sorted from adult (12-month-old, n = 5) and aged (24-month-old, n = 4) mouse brains and analyzed by scRNA-seq. Combined (top, 8,809 cells) and separate visualization of cells from adult (middle, 4,338 cells) and aged (bottom, 4,471 cells) brains are displayed. b, Heatmap of top 10 cluster-specific genes. The color scale is based on a z-score distribution from -2 (purple) to 2 (yellow). c, Heatmaps of differentially expressed genes comparing cells isolated from adult and aged brains across all clusters (left) or within cluster 2 (right) as identified in panel a. d, Contribution of the samples to each cluster is displayed in percent and e, absolute numbers extrapolated to total cells per brain. f, Heatmap of differentially expressed genes between cluster 1 and cluster 3 as annotated in panel a. g, Violin plots of Lag3 (top) and Gzmb (bottom) gene expression in clusters 1 to 5. IST, interferon-stimulated T cells; TEFF, effector T cells; TCM, central memory T cells.
